Today, the organizational culture variable is the main focus of analysis in many organizational studies. In the last years, this construct, has found special status in the field of sport management researches. This study aims to validate and investigate the psychometric properties of organizational culture assessment instrument (OCAI) based on the competing values framework model (CVF) in Iranian sport organizations (federations). The methodology of the present research is based on a correlation study (A factor analysis type of covariant matrix or correlation through principle components analysis method). The statistical population includes 52 sport federations (703 sport experts). In confirmatory factor analysis (CFA) method, the number of samples was determined according to the number of variables and 253 subjects (83.77%) were selected in a stratified random sampling method as final samples. The data were collected through OCAI and the construct validity, also, analyzed using LISREL 8.70 software and a CFA method.  The results obtained showed that OCAI questionnaire was a multi-dimensional construct and the four OCAI factors were confirmed to be as cultural profiles: Clan (6 items), Adhocracy (6 items), Market (6 items) and Hierarchy (6 items). The other result, confirmed the OCAI reliability suitability by computing internal consistency through cronbach &alpha test (r=0/86). Considering the confirmation of psychometric properties of OCAI and cultural profiles in factor analysis, organizational culture assessment instrument (OCAI) based on the competing values framework model (CVF) seems to be an appropriate instrument and theoretical framework for assessing the organizational culture in Iranian sports organizations (federations). Therefore, it is suggested that sport management researchers attempt to assess the organizational culture by using OCAI questionnaire in Iranian sport organizations.