The aim of this study was to measure the validity and reliability of perceived motivational climate in sport questionnaire (PMCSQ-2) for Newton and colleagues [2000[. For this purpose, random participants from 553 athletes 13 to 23 years were selected to complete the questionnaire. After applying the translate-retranslate technique, content validity ratio (CVR) and content validity index (CVI) confirmed by 10 Panels (CVR=0.66, CVI=0.97.For determining the construct validity, confirmatory factor analysis (CFA) based on structural equations was used, internal consistency of questionnaire was achieved by Cranach's alpha and Pearson correlation coefficient under test-retest method to study reliability of questionnaire. Results showed that the first model of PMCSQ-2(33 question (, wasn’t able to support the favorable fit and thus eliminated 6 problematic questions. Thereby the final model with 27 questions was included task-oriented climate (TOC) (effort/improvement, important role and cooperative learning) and ego-oriented climate (EOC) (unequal recognition and punishment of mistakes). Hereby, the Persian PMCSQ-2 (PPMCSQ-2) had acceptable fit index (RMSEA= 0.054, CFI= 0.9). Also, Internal consistency was acceptable (TOC=0.827, EOC=0.726 and PPMCSQ-2=0.618) and reliability of PPMCSQ-2(r=0.73) was affirmed. Therefore, PPMCSQ-2 can be used as a valid and reliable instrument to assess motivational climate in the Iranian athletes with the age range of 13 to 23 years.